hepatoma cells huh7 5  (ATCC)

Journal: PLoS ONE

Article Title: Development, Characterization and Application of Monoclonal Antibodies against Brazilian Dengue Virus Isolates

doi: 10.1371/journal.pone.0110620

Figure Lengend Snippet: Vero E6 cells were infected with WNV (A), whereas Huh7.5 cells were infected with YFV and SLEV (B). Cells were fixed in methanol:acetone and stained with mAbs, followed by Alexa-Fluor 488-conjugated anti-mouse immunoglobulin. Monoclonal antibody 4G2 and a non-correlated anti-hantavirus mAb (572/7A) were used as positive and negative controls, respectively. Images were obtained with a Leica AF6000 Modular System. Scale bars are 30 µm.

Article Snippet: Human-derived hepatoma cells (Huh7.5) (ATCC PTA-8561) and Vero E6 cells (Sigma, 85020206) were maintained in Dulbecco’s Modified Eagle Medium/Nutrient Ham F12 (DMEM F12 – Gibco) with 10% FBS, 14.0 mM sodium bicarbonate and antibiotics (100 IU/ml penicillin, 100 µg/ml streptomycin) at 37°C in a 5% CO 2 atmosphere.

Techniques: Infection, Staining

Journal: International Journal of Molecular Sciences

Article Title: The Polyphenol-Rich Extract from Psiloxylon mauritianum, an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection

doi: 10.3390/ijms20081860

Figure Lengend Snippet: The P. mauritianum extract inhibits the infection of four DENV clinical isolates representative of the four serotypes. Four DENV serotypes (DENV-1, 2, 3 and 4) were pre-incubated with the P. mauritianum extract for 1 h at 37 °C. ( A ) Huh7.5 cells were infected for 48 h with DENV at the different MOI 0.2, 2, 0.5 and 2 for DENV 1–4, respectively. Recombinant IFN-α 2A (200 IU mL −1 ) was added 2 h post-infection and used as a positive control. The percentage of E positive cells was evaluated using the Operetta High-Content Imaging System (PerkinElmer). The results are the means ± SD of three independent experiments. ( B ) The residual infectious particles were titrated in C6/36 cells using a foci-forming immunodetection assay. The data represent the means ± SD from three independent experiments. A one-way ANOVA and Dunnett’s test for multiple comparisons were used for the statistical analysis (** p < 0.01; *** p < 0.001; **** p < 0.0001).

Article Snippet: Vero cells (ATCC, CCL-81) and human-derived Huh7.5 hepatoma cells (ATCC, PTA-8561TM; Manassas, VA, USA) were cultured in an Eagle minimum essential medium (MEM) supplemented with a 5% heat-inactivated Fetal Bovine Serum (FBS), 2 mmol L −1 L-Glutamine, 1 mmol L −1 sodium pyruvate, 100 U mL −1 of penicillin, 0.1 mg mL −1 of streptomycin and 0.5 µg mL −1 of fungizone (Amphotericin B) (PAN Biotech, Aidenbach, Germany) under a 5% CO 2 atmosphere at 37 °C.

Techniques: Infection, Incubation, Recombinant, Positive Control, Imaging, Immunodetection

Journal: Scientific Reports

Article Title: The citrus flavanone naringenin impairs dengue virus replication in human cells

doi: 10.1038/srep41864

Figure Lengend Snippet: Naringenin half maximal inhibitory concentration (IC 50 ) and selectivity index (SI) for anti-DENV activity in Huh7.5 cells.

Article Snippet: The human-derived Huh7.5 hepatoma cell line (ATCC PTA-8561) was grown in Dulbecco’s Modified Eagle Medium/nutrient mixture F-12 (D-MEM/F-12; Gibco, Grand Island, NY, USA) supplemented with 100 IU/μg/mL penicillin/streptomycin (Gibco, Grand Island, NY, USA) and 10% FBS at 37 °C in a humidified, 5% CO 2 controlled atmosphere.

Techniques: Concentration Assay, Activity Assay